一期Nature，两篇国产文！赞！

Androgenetic haploid embryonic stem cells produce live transgenic mice Nature 490, 7420 (2012). doi:10.1038/nature11435 Authors: Wei Li, Ling Shuai, Haifeng Wan, Mingzhu Dong, Meng Wang, Lisi Sang, Chunjing Feng, Guan-Zheng Luo, Tianda Li, Xin Li, Libin Wang, Qin-Yuan Zheng, Chao Sheng, Hua-Jun Wu, Zhonghua Liu, Lei Liu, Liu Wang, Xiu-Jie Wang, Xiao-Yang Zhao & Qi Zhou Haploids and double haploids are important resources for studying recessive traits and have large impacts on crop breeding, but natural haploids are rare in animals. Mammalian haploids are restricted to germline cells and are occasionally found in tumours with massive chromosome loss. Recent success in establishing haploid embryonic stem (ES) cells in medaka fish and mice raised the possibility of using engineered mammalian haploid cells in genetic studies. However, the availability and functional characterization of mammalian haploid ES cells are still limited. Here we show that mouse androgenetic haploid ES (ahES) cell lines can be established by transferring sperm into an enucleated oocyte. The ahES cells maintain haploidy and stable growth over 30 passages, express pluripotent markers, possess the ability to differentiate into all three germ layers in vitro and in vivo, and contribute to germlines of chimaeras when injected into blastocysts. Although epigenetically distinct from sperm cells, the ahES cells can produce viable and fertile progenies after intracytoplasmic injection into mature oocytes. The oocyte-injection procedure can also produce viable transgenic mice from genetically engineered ahES cells. Our findings show the developmental pluripotency of androgenentic haploids and provide a new tool to quickly produce genetic models for recessive traits. They may also shed new light on assisted reproduction.

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这篇貌似饶教授的名字拼错了！（更正：误会，其实不是饶教授）

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Posted on: Wednesday, 17 October 2012 11:00 AM
Author: Linfeng Sun
Subject: Crystal structure of a bacterial homologue of glucose transporters GLUT1–4

Crystal structure of a bacterial homologue of glucose transporters GLUT1–4 Nature 490, 7420 (2012). doi:10.1038/nature11524 Authors: Linfeng Sun, Xin Zeng, Chuangye Yan, Xiuyun Sun, Xinqi Gong, Yu Rao & Nieng Yan Glucose transporters are essential for metabolism of glucose in cells of diverse organisms from microbes to humans, exemplified by the disease-related human proteins GLUT1, 2, 3 and 4. Despite rigorous efforts, the structural information for GLUT1–4 or their homologues remains largely unknown. Here we report

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